Chimeric RNA/DNA oligonucleotides have been shown to promote single nucleotide
exchange in genomic DNA. A chimeric molecule was designed to introduce an A to C
nucleotide conversion at the Ser 365 position of the rat factor IX gene. The oligonucleotides
were encapsulated in positive, neutral, and negatively charged liposomes containing
galactocerebroside or complexed with lactosylated polyethyleneimine. The formulations
were evaluated for stability and efficiency in targeting hepatocytes via the asialoglycoprotein …