Circulating IgA-and IgG-class antigliadin antibodies in dermatitis herpetiformis detected by enzyme-linked immunosorbent assay
E Vainio, K Kalimo, T Reunala, M Viander… - Archives of …, 1983 - Springer
E Vainio, K Kalimo, T Reunala, M Viander, T Palosuo
Archives of Dermatological Research, 1983•SpringerA sensitive and technically simple enzymelinked immunosorbent assay (ELISA) was
developed to demonstrate circulating IgA-and IgG-class antibodies to gliadin, a component
of wheat gluten. Serum samples from 24 patients with dermatitis herpetiformis (DH), 5 with
coeliac disease (CD) and 75 normal controls were analysed. Antigliadin antibodies (AGA) of
the IgA class were detected in 71% of DH patients, all of the CD patients and 19% of the
controls. IgG-AGA was found in over 90% of DH patients and controls and in all of the CD …
developed to demonstrate circulating IgA-and IgG-class antibodies to gliadin, a component
of wheat gluten. Serum samples from 24 patients with dermatitis herpetiformis (DH), 5 with
coeliac disease (CD) and 75 normal controls were analysed. Antigliadin antibodies (AGA) of
the IgA class were detected in 71% of DH patients, all of the CD patients and 19% of the
controls. IgG-AGA was found in over 90% of DH patients and controls and in all of the CD …
Summary
A sensitive and technically simple enzymelinked immunosorbent assay (ELISA) was developed to demonstrate circulating IgA- and IgG-class antibodies to gliadin, a component of wheat gluten. Serum samples from 24 patients with dermatitis herpetiformis (DH), 5 with coeliac disease (CD) and 75 normal controls were analysed. Antigliadin antibodies (AGA) of the IgA class were detected in 71% of DH patients, all of the CD patients and 19% of the controls. IgG-AGA was found in over 90% of DH patients and controls and in all of the CD patients. The mean ELISA values of both IgA- and IgG-class AGA were significantly higher in DH patients than in the controls. The occurrence of circulating IgA-class AGA is compatible with the hypothesis that these antibodies can be deposited in the skin, e.g. as immune complexes, or due to cross-reactivity of gliadin and dermal reticulin.
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