Human TRIM gene expression in response to interferons
L Carthagena, A Bergamaschi, JM Luna, A David… - PloS one, 2009 - journals.plos.org
PloS one, 2009•journals.plos.org
Background Tripartite motif (TRIM) proteins constitute a family of proteins that share a
conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5α in
primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral
activities and innate immunity. Principal Findings To test if TRIM genes are up-regulated
during antiviral immune responses, we performed a systematic analysis of TRIM gene
expression in human primary lymphocytes and monocyte-derived macrophages in response …
conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5α in
primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral
activities and innate immunity. Principal Findings To test if TRIM genes are up-regulated
during antiviral immune responses, we performed a systematic analysis of TRIM gene
expression in human primary lymphocytes and monocyte-derived macrophages in response …
Background
Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5α in primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral activities and innate immunity.
Principal Findings
To test if TRIM genes are up-regulated during antiviral immune responses, we performed a systematic analysis of TRIM gene expression in human primary lymphocytes and monocyte-derived macrophages in response to interferons (IFNs, type I and II) or following FcγR-mediated activation of macrophages. We found that 27 of the 72 human TRIM genes are sensitive to IFN. Our analysis identifies 9 additional TRIM genes that are up-regulated by IFNs, among which only 3 have previously been found to display an antiviral activity. Also, we found 2 TRIM proteins, TRIM9 and 54, to be specifically up-regulated in FcγR-activated macrophages.
Conclusions
Our results present the first comprehensive TRIM gene expression analysis in primary human immune cells, and suggest the involvement of additional TRIM proteins in regulating host antiviral activities.
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