[HTML][HTML] Characterization of the S-denitrosation activity of protein disulfide isomerase
S-Nitrosoglutathione (GSNO) denitrosation activity of recombinant human protein disulfide
isomerase (PDI) has been kinetically characterized by monitoring the loss of the S–NO
absorbance, using a NO electrode, and with the aid of the fluorogenic NO x probe 2, 3-
diaminonaphthalene. The initial rates of denitrosation as a function of [GSNO] displayed
hyperbolic behavior irrespective of the method used to monitor denitrosation. The K m
values estimated for GSNO were 65±5 μm and 40±10 μm for the loss in the S–NO bond and …
isomerase (PDI) has been kinetically characterized by monitoring the loss of the S–NO
absorbance, using a NO electrode, and with the aid of the fluorogenic NO x probe 2, 3-
diaminonaphthalene. The initial rates of denitrosation as a function of [GSNO] displayed
hyperbolic behavior irrespective of the method used to monitor denitrosation. The K m
values estimated for GSNO were 65±5 μm and 40±10 μm for the loss in the S–NO bond and …