Impaired control of multiple viral infections in a family with complete IRF9 deficiency
MB García-Morato, AC Apalategi… - Journal of Allergy and …, 2019 - jacionline.org
Cell lines in which IRF9 expression was targeted by using CRISPR/Cas9 technology were
prepared to establish a direct relationship between IRF9 deficiency and susceptibility to
virus infection. Impaired induction of ISGs after IFN-a treatment was noted in cells in which
IRF9 expression was reduced by 2 independent guide RNAs (Fig 2, A). IRF9 silencing
decreased cellular control of viral replication (Fig 2, B), especially when cells were
pretreated with IFN-a (Fig 2, C). In contrast, transfection of patients' cells with wild-type, but …
prepared to establish a direct relationship between IRF9 deficiency and susceptibility to
virus infection. Impaired induction of ISGs after IFN-a treatment was noted in cells in which
IRF9 expression was reduced by 2 independent guide RNAs (Fig 2, A). IRF9 silencing
decreased cellular control of viral replication (Fig 2, B), especially when cells were
pretreated with IFN-a (Fig 2, C). In contrast, transfection of patients' cells with wild-type, but …