Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa
AS Magaret, A Wald, ML Huang, S Selke… - Journal of clinical …, 2007 - Am Soc Microbiol
AS Magaret, A Wald, ML Huang, S Selke, L Corey
Journal of clinical microbiology, 2007•Am Soc MicrobiolIn 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA.
Recently, we determined an optimal positivity criterion based on research specimens and a
dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-
fold lower than our previous cutoff, minimizes misclassification.
Recently, we determined an optimal positivity criterion based on research specimens and a
dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-
fold lower than our previous cutoff, minimizes misclassification.
Abstract
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
American Society for Microbiology