Simplified method of the growth of human tumor infiltrating lymphocytes in gas-permeable flasks to numbers needed for patient treatment
J Jin, M Sabatino, R Somerville, JR Wilson… - Journal of …, 2012 - journals.lww.com
Adoptive cell therapy of metastatic melanoma with autologous tumor infiltrating lymphocytes
(TIL) is clinically effective, but TIL production can be challenging. Here we describe a
simplified method for initial TIL culture and rapid expansion in gas-permeable flasks. TIL
were initially cultured from tumor digests and fragments in 40 mL capacity flasks with a 10
cm 2 gas-permeable silicone bottom, G-Rex10. A TIL rapid expansion protocol (REP) was
developed using 500 mL capacity flasks with a 100 cm 2 gas-permeable silicone bottom, G …
(TIL) is clinically effective, but TIL production can be challenging. Here we describe a
simplified method for initial TIL culture and rapid expansion in gas-permeable flasks. TIL
were initially cultured from tumor digests and fragments in 40 mL capacity flasks with a 10
cm 2 gas-permeable silicone bottom, G-Rex10. A TIL rapid expansion protocol (REP) was
developed using 500 mL capacity flasks with a 100 cm 2 gas-permeable silicone bottom, G …
