Biotin-labeled hairpin oligonucleotides: probes to detect double-strand breaks in DNA in apoptotic cells.
VV Didenko, JR Tunstead… - The American journal of …, 1998 - ncbi.nlm.nih.gov
VV Didenko, JR Tunstead, PJ Hornsby
The American journal of pathology, 1998•ncbi.nlm.nih.govHairpin oligonucleotides were synthesized with stems ending in a double-stranded
structure, which can be ligated to double-strand breaks in DNA, and with loops that contain
nucleotides modified by the attachment of biotin. These probes specifically and sensitively
detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to
areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was
obtained when all available 3'DNA ends were labeled by terminal transferase. In principle …
structure, which can be ligated to double-strand breaks in DNA, and with loops that contain
nucleotides modified by the attachment of biotin. These probes specifically and sensitively
detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to
areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was
obtained when all available 3'DNA ends were labeled by terminal transferase. In principle …
Abstract
Hairpin oligonucleotides were synthesized with stems ending in a double-stranded structure, which can be ligated to double-strand breaks in DNA, and with loops that contain nucleotides modified by the attachment of biotin. These probes specifically and sensitively detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was obtained when all available 3'DNA ends were labeled by terminal transferase. In principle, hairpin oligonucleotide probes can be designed with any type of 3'or 5'overhang complementary to double-strand DNA termini being detected.
ncbi.nlm.nih.gov
